Иммунофлуоресценция

Fluorescent Signal Amplification

Biocare’s Signal Amplification Reagent increases fluorescent signals 3 to 5-fold when used in combination with DyLight immunofluorescent detection kits. This increased sensitivity is perfect for identification of low abundance proteins as well as routine work. Amplify your staining intensity for nuclear, cell membrane, and cytoplasmic antigens with Biocare’s Fluorescence Enhancement Probe technology. For use on human tissue only.

• 3 to 5-fold Signal amplification with Biocare DyLight Detection Kits
• Superior to FITC and TRITC
• Multiplex detection protocols

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Fluorescent Signal Preservation

Biocare’s anti-fade mountants and antibody diluents deliver superior performance and signal preservation. This innovative, liquid-based, anti-fade mounting medium is especially designed for the permanent preservation of fluorescent specimens. The protease-free antibody diluents stabilize fluorescent dyes for up to one month in a prediluted format.

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Fluorescent DyLight™ Detection Kits

Biocare DyLight fluors are a family of high-intensity, photostable, conjugated fluorescent dyes for immunofluorescent studies. DyLight dyes exhibit comparable fluorescence intensity and photostability to Alexa Fluor® and CyDyeTM dyes. Biocare fluors remain highly fluorescent over a broad pH range (pH 4-9). Narrow emission spectra enable multi-color detection by minimizing cross talk.

DyLight detection is provided as concentrated secondary anti-mouse or anti-rabbit antibodies. Biocare’s fluorescent signal amplification reagent may be added to the standard DyLight detection kit to greatly increase the signal.

Increased photostability allows more time for collaborative interpretation of results.

• Proven on paraffin-embedded (FFPE) and frozen tissues as well as cell culture preparations
• Spectrum and photosensitivity equivalent to Alexa Fluor® 488, 546, and 555
• Room temperature photostability of > 30 days. Experiments may be performed in the light
• Advanced mountants simplify slide handling compared to PBS glycerol, while retaining non-toxic attributes
• Unique animal tissue blocking and antigen retrieval products reduce background
• Ideal for free-floating sections, allowing thicker tissue sections (40 microns) than frozen or FFPE

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DyLight™ is a trademark of Pierce Biotechnology, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
CyDye™ is a trademark of Amersham Biosciences.